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Laboratory diagnosis of pheochromocytoma
Chlupáčová, Tereza ; Bílek, Radovan (advisor) ; Švandová, Ivana (referee)
1 Phaeochromocytomas are tumors predominantly rare to chromaffin tissue in adrenal glands' medulla. Tumors in the extra-adrenal chromaffin tissue are called paragangliomas. Phaeochromocytomas cause secretion of high amounts of catecholamines, which can lead to fatal consequences if not medically treated. The aim of this thesis is to summarize the so far known facts about this severe disease in a written review. The first part of the study focuses on issues of phaeochromocytoma research and lists general information about these tumors and their clinical manifestations. The importance of genetic influence in connection with treatment strategies for patients with phaeochromocytoma is also discussed. The second part of the thesis summarizes procedures and methods used in laboratory diagnostics of phaeochromocytomas. The conclusion briefly describes the possibilities of tumor localization using imaging technologies and procedures of subsequent treatment of patients with phaeochromocytoma.
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Laboratory diagnosis of pheochromocytoma
Chlupáčová, Tereza ; Bílek, Radovan (advisor) ; Švandová, Ivana (referee)
1 Phaeochromocytomas are tumors predominantly rare to chromaffin tissue in adrenal glands' medulla. Tumors in the extra-adrenal chromaffin tissue are called paragangliomas. Phaeochromocytomas cause secretion of high amounts of catecholamines, which can lead to fatal consequences if not medically treated. The aim of this thesis is to summarize the so far known facts about this severe disease in a written review. The first part of the study focuses on issues of phaeochromocytoma research and lists general information about these tumors and their clinical manifestations. The importance of genetic influence in connection with treatment strategies for patients with phaeochromocytoma is also discussed. The second part of the thesis summarizes procedures and methods used in laboratory diagnostics of phaeochromocytomas. The conclusion briefly describes the possibilities of tumor localization using imaging technologies and procedures of subsequent treatment of patients with phaeochromocytoma.
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Determination of adrenaline and noradrenaline in plasma by HPLC
Kučerová, Tereza ; Klapková, Eva (advisor) ; Kotaška, Karel (referee)
The target of this bacalary work was to validate the metod for determination of adrenaline and noradrenaline in plasma by high performance liquid chromatography with electrochemical (coulometric) detection. The metod was first necessary to optimize for the coulometric detection because it was used the Bio-Rad kit, which was designed to determine with amperometric detection. The principle of this metod is the chromatographic separation of substances with subsequent oxidation of the determined analyte passes through the analytic cell with coulometric detection. The part of the analytical procedure is the solid phase extraction. The part of this work was the optimization of the metod for the coulometric detector. For the analysis were selected following potentials: channel 1 - 350 mV with range 100 nA, channel 2 - 0 mV with range 50 nA, guard channel 0 mV with range 50 nA. Furthermore, the chromatographic metod was fully validated (accuracy, precision, limit of detection, robustness). In the conclusion was made interlaboratory comparison of results of 42 patients samples with those obtained in the hospital Šternberk.
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